Category Archives: trpml

Yu Z., Willmarth N. induces endogenous cells factor manifestation in MCF-7 cells, and overexpression of miR-19 down-regulates cells factor CCK2R Ligand-Linker Conjugates 1 manifestation in MDA-MB-231 cells. RT-PCR analysis using cDNA made from Ago2-immunoprecipitated RNA samples confirmed that Ago2 binds preferentially to cells element 3-UTR in MCF-7 cells, as compared with MDA-MB-231 cells, consistent with the observation that miR-19 levels are higher in MCF-7 cells. luciferase CCK2R Ligand-Linker Conjugates 1 reporter vector (Promega, Madison, WI), and 10 l of FuGENE HD transfection reagent (Roche Applied Technology) in 2 ml of serum-free medium was added. After 5 h of incubation, the medium was replaced with 2 ml of RPMI or DMEM with 10% fetal bovine serum, and cells were incubated immediately. The cells then were lifted and plated into a 24-well plate at a denseness of 250,000 per well. Luminescence Rabbit Polyclonal to Retinoic Acid Receptor beta was measured 72 h…

Read more

1/1