Category Archives: Tryptophan Hydroxylase

´╗┐Cancer Cell. phase and both apoptosis and autophagy. Targeting Akt as a key protein of PI3K/Akt/mTOR pathway with multiple drugs might represent a new and promising pharmacological strategy for treatment of T-ALL patients. treatment with MK-2206, GSK690693 and Perifosine could lead to a modulation of PI3K/Akt/mTOR pathway, we checked the phosphorylation status of key components of this signaling cascade in our panel of more responsive cell lines. In particular we analyzed p-Akt, its downstream target, GSK3 /, and TTP-22 the ribosomal protein S6 kinase, readout of mTORC1 activity, after 30 min of drugs exposure. GSK690693 and Perifosine were used at 1/2 of the IC50 concentration, whereas MK-2206 was used at 1/5 of IC50, since half of MK-2206 IC50 concentration was enough to completely abolish the Ser 473 Akt phosphorylation already at 30 minutes. Akt phosphorylation was affected in different ways by single drug administration: in all cell lines MK-2206 very…

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